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Table 1 Frequency of AM detachment phenotypes in Cg25C mutants

From: Distinct functions of the laminin β LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila

Genotype

Fraction of abnormal embryo half-sides

 

19°C

25°C

29°C

Cg25C S3064 /CyO

n.d.

0% (n = 58)

n.d.

control/Df(2L)Exel7022

n.d.

1.5% (n = 66)

1.8% (n = 56)

Cg25C S0120 /Df(2L)Exel7022

40% (n = 15)

79.6% (n = 142)

73.3% (n = 30)

Cg25C S0791 /Df(2L)Exel7022

0% (n = 38)

87.1% (n = 62)

93.3% (n = 30)

Cg25C S1348 /Df(2L)Exel7022

6.5% (n = 31)

76.6% (n = 94)

93.3% (n = 30)

Cg25C S2186 /Df(2L)Exel7022

3.4% (n = 59)

15.3% (n = 85)

83.3% (n = 30)

Cg25C S3064 /Df(2L)Exel7022

32.4% (n = 34)

99% (n = 104)

89.2% (n = 74)

Cg25C DTS-L3 /Df(2L)Exel7022

8.1% (n = 34)

100% (n = 88)

88.2% (n = 76)

Cg25C S3064 /Cg25C DTS-L3

10% (n = 30)

100% (n = 72)

100% (n = 75)

Df(2L)Exel7022/Df(2L)Exel7022

73.3% (n = 15)

54% (n = 87)

63.5% (n = 63)

  1. Embryos were analyzed for the position of AM attachments at late stage 17 (trachea become clearly visible) after development at the indicated temperature. Embryo half-sides in which at least one of the seven AMs showed an increased distance between its dorsal end and the DV were counted as abnormal (n = total number of scored half-sides; control = S-18a-13b-16b.1 with GFP/RFP reporter constructs; analogous 3rd chromosome reporters were crossed in for analysis of genotypes lacking 2nd chromosome reporters).
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BMC Developmental Biology

ISSN: 1471-213X

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