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Table 1 Frequency of AM detachment phenotypes in Cg25C mutants

From: Distinct functions of the laminin β LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila

Genotype Fraction of abnormal embryo half-sides
  19°C 25°C 29°C
Cg25C S3064 /CyO n.d. 0% (n = 58) n.d.
control/Df(2L)Exel7022 n.d. 1.5% (n = 66) 1.8% (n = 56)
Cg25C S0120 /Df(2L)Exel7022 40% (n = 15) 79.6% (n = 142) 73.3% (n = 30)
Cg25C S0791 /Df(2L)Exel7022 0% (n = 38) 87.1% (n = 62) 93.3% (n = 30)
Cg25C S1348 /Df(2L)Exel7022 6.5% (n = 31) 76.6% (n = 94) 93.3% (n = 30)
Cg25C S2186 /Df(2L)Exel7022 3.4% (n = 59) 15.3% (n = 85) 83.3% (n = 30)
Cg25C S3064 /Df(2L)Exel7022 32.4% (n = 34) 99% (n = 104) 89.2% (n = 74)
Cg25C DTS-L3 /Df(2L)Exel7022 8.1% (n = 34) 100% (n = 88) 88.2% (n = 76)
Cg25C S3064 /Cg25C DTS-L3 10% (n = 30) 100% (n = 72) 100% (n = 75)
Df(2L)Exel7022/Df(2L)Exel7022 73.3% (n = 15) 54% (n = 87) 63.5% (n = 63)
  1. Embryos were analyzed for the position of AM attachments at late stage 17 (trachea become clearly visible) after development at the indicated temperature. Embryo half-sides in which at least one of the seven AMs showed an increased distance between its dorsal end and the DV were counted as abnormal (n = total number of scored half-sides; control = S-18a-13b-16b.1 with GFP/RFP reporter constructs; analogous 3rd chromosome reporters were crossed in for analysis of genotypes lacking 2nd chromosome reporters).