Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line

Padmakumar, VC; Masiuk, Katelyn E; Luger, Dror; Lee, Christina; Coppola, Vincenzo; Tessarollo, Lino; Hoover, Shelley B; Karavanova, Irina; Buonanno, Andres; Simpson, R Mark; Yuspa, Stuart H

doi:10.1186/1471-213X-14-24

Table 1 CLIC4 expression in adult tissue structures and cell types

From: Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line

CLIC4-GFP-KI mouse tissue	Major cell type or structure labeled	CLIC4-GFP labeling homozygote	CLIC4-GFP labeling heterozygote
Liver	Hepatocytes	+	0/+
	Sinusoidal lining cells	+	0/+
	Bile ductules	+	0/+
	Arteriolar smooth muscle*	+	+
	Venules*	+	+
Lung	Alveolar septae endothelium	+	+
	Alveolar pneumocytes	0	0
	Venular endothelium	+	+
	Bronchiolar arteriolar smooth muscle*	0	0
	Pulmonary arteriolar and venular smooth muscle	+	+
	Bronchiolar epithelium	0	0
	Bronchiolar smooth muscle	0/+	0/+
Kidney	Glomerular tufts- endothelial cells	+	+
	Glomerular visceral podocytes	0	0
	Proximal convoluted tubules	+	0/+
	Distal convoluted tubules	0	0
	Medullary endothelium	+	0/+
	Medullary collecting ducts	0	0
Spleen	Red pulp tissue - hematopoietic cells	+	0
	White pulp -Individual cells, nonlymphoid monocytoid †	+	0
Heart	Cardiomyocytes	Diffuse labeling +	0/+
	Coronary and pulmonary artery and vein smooth muscle	+	0/+
Thymus	Thymic medullary epithelial cells	+	+
	Medullary thymocytes	0/+	0/+
Trachea	Tracheal mucosa	0	0
	Perichondrium	0/+	0
	Chondrocytes	+	0/+
Pancreas	Islet cells	Heterogeneous: +	0
	Exocrine	+	0/+
	Ductules	+	0/+
Stomach	Parietal cells	na	0
	Chief cells	na	0
	Smooth muscle	na	0/+
Esophagus		0	0
Jejunum	Villus epithelium	na	0
	Lamina proprial endothelium	na	0/+
	Smooth muscle	na	0/+
Skeletal Muscle	Myocytes	0	0
Urinary Bladder	Mucosal transitional epithelium	0	0
	Smooth muscle	0	0
Ureter	Mucosal transitional epithelium	0	0
	Smooth muscle	0	0
Urethra	Mucosal transitional epithelium	Variable labeling +	0
Testis	Spermatagonia type A	Variable labeling +	na
	Spermatogonia type B	+	na
	Sertoli cells	0/+	na
	Interstitial cells of Leydig	0/+	na
	Vas deferens	0	0
Accessory Sex Glands	Anterior prostate	0	0
	Dorsal prostate	0	0
	Lateral and ventral prostate	+	0/+
	Coagulating gland	+	0/+
	Bulbourethral gland	+	na
Adrenal Gland**		na	+
Thyroid Gland	Follicular epithelium	na	Variable labeling 0/+

The table lists major cell types and structures labeled by anti-GFP staining in both homozygous and heterozygous mice. SCORING: 0 = Negative, + = Cell type/tissue positive, 0/+ = Heterogeneous immunolabeling (only some cells label) in homozygous; weak immunolabeling in heterozygous. NOTES: *Vascular smooth muscle myocytes and endothelium were uniformly positive in all tissues unless otherwise noted (homozygous). na = Tissue not available. † Cells morphologically appear to be dendritic cells. **Outer zone of adrenal medullary pheochromocytes subjacent to the corticomedullary junction. Cell types not listed within a given tissue were negative for expression except for vascular endothelium which was labeled throughout all tissues in the homozygous but not universally so in the heterozygous.

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ISSN: 1471-213X

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