Figure 4

CLIC4 expression in organs from 4 week old WT and homozygous CLIC4-GFP mice. The left panels show GFP staining representing CLIC4 expression in liver (A-B), lung (C-D), pancreas (E-F) and testis (G-H). The right panels are the corresponding organs from WT control mice treated with anti-GFP, indicating no specific GFP expression. (A-B): Most liver cell types exhibit expression (see also Table 1 for additional clarification). Arrow indicates labeling of bile ductular epithelium. (C-D): CLIC4 is evident in pulmonary endothelia including those lining blood vessels and within the alveolar septae (arrowheads). The alveolar pneumocytes (arrows, and see inset photomicrograph) and bronchiolar epithelia lack expression. (E-F): Pancreatic islet cells are heterogeneously immuno-labeled while exocrine acinar cells are uniformly positive. Pancreatic ductular epithelia are labeled (arrow) (see also Table 1) (arrow = duct). (G-H): Type A spermatogonia exhibit variable labeling at the margins of seminiferous tubules, while adluminal type B spermatogonia and spermatocytes express CLIC4, although the latter cells have variable and less pronounced signal. Sertoli cells heterogeneously express CLIC4-GFP with few cells labeling. Interstitial cells are also heterogeneously labeled, (see also Table 1). Hematoxylin counterstain. Bar = 50 um.