Figure 1

Generation of CLIC4-GFP knock-in mouse. Panel A is the schematic of the strategy used for GFP knock-in vector. B, D and F are light images and C, E and G are fluorescent images. (B-C): light and fluorescent images of tails from adult WT and CLIC4-GFP mice. (D-G): light and fluorescent images of WT and CLIC4-GFP new born pups. (H): Southern blot from embryonic stem cells used for blastocyst injection; (I): Immunoblot of protein lysates from WT and homozygous CLIC4-GFP skin using antibodies against CLIC4, GFP and β-actin; (J): qPCR analysis of spleen lysates from WT and CLIC4-GFP heterozygous (WT/KI) or homozygous (KI/KI) mice probed for CLIC4 N-terminus.