Figure 5

Mitochondrial reactive oxygen species ( mtROS) production in bone marrow mesenchymal stem cells (bmMSCs) after exposure to 0, 1, 5 and 10 ng/ mL TGF -β1 for 24 hours. A. MitoSOX™ Red Indicator staining shows mtROS in bmMSCs after exposure to 0, 1, 5 and 10 ng/mL TGF-β1 for 24 hours. B. Quantification of fluorescence density of mtROS. C. Western blot assay shows expression of superoxide dismutase 2 (SOD2) in bmMSCs after exposure to 0, 1, 5 and 10 ng/mL TGF-β1 for 24 hours. Bar graphs represent mean ± SD (4 independent experiments/group). *P < 0.05 vs. control.