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Figure 6 | BMC Developmental Biology

Figure 6

From: Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6

Figure 6

ELT-6 interacts with pJW3.9 through a GATA binding site. A) Yeast strains containing HIS3 and lacZ reporters transformed with empty vector (Control) or ELT-6::GAL4AD plasmids (ELT-6) were diluted and replica plated to control (left), 10 mM 3aminotriazole (3AT, middle), and XGal (right) plates. Reporters had inserts of either JW3.9 (top) or JW3.9 in which GATA binding site S1 was mutated from TGATAA to GGTACC (‘Mut’ bottom). Mutation of the GATA site abolishes the interaction with ELT-6 based on lack of growth on 3AT and lack of blue color on XGal.; B) Increasing concentrations of ELT-6 interact with a 40 bp fragment around GATA site S1 (lanes 2-6). Mutation of the GATA site S1 from TGATAA to GGTACC (M1) abolishes interaction with ELT-6 (lane 8); C) 50 ng of cold wild type site S1 (W) can compete effectively for binding of ELT-6 to S1 (compare lanes 2 and 3). Mutation of GATA site S1 (M1) reduces but does not abolish the ability to compete (lane 4). Mutation of both site S1 and a second GATA site on the oligonucleotide (M2) drastically reduces the ability of the oligonucleotide to compete for ELT-6 binding (lane 5).

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BMC Developmental Biology

ISSN: 1471-213X

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