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Figure 2 | BMC Developmental Biology

Figure 2

From: Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6

Figure 2

Seven transcription factors affect lin-39::GFP expression in the VPCs at early L3 stage. GFP expression in the VPCs P5.p - P8.p from smg-1; deIS4 (lin-39::GFP) animals treated for RNAi of individual transcription factors identified in yeast (panels B - H), or given control RNAi (empty vector; panel A). Early L3 stage animals are shown; anterior is left, ventral is down. These animals also express ajm-1::GFP, which outlines cell junctions of hypodermal cells and the pharynx (bright staining in the anterior seen in most panels). RNAi for lin-26, bed-3, and tbx-9 causes severe embryonic lethal and larva arrest phenotypes, so RNAi for these genes was performed by feeding newly-hatched L1 larvae on RNAi bacterial lawns and examining GFP expression in these same animals at the L3 stage. For all other genes, RNAi treatment was carried out on P0 animals, and their F1 progeny were examined as L3 animals. All pictures were taken under the same exposure.

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BMC Developmental Biology

ISSN: 1471-213X

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