Figure 2

mRNA expression of 12 genes detected by microarray and qRT-PCR. The mRNA relative expression of 12 selected genes. (A) Amelogenin, membrane-associated ring finger 6 (MARCH6), isolate X269 mitochondrion, DSPP600 (DSPP), enamelin precursor, and caveolin were up-regulated at E60 compared with those at E45 in the microarray. (B) Ribosomal protein L7, eukaryotic translation elongation factor 1 alpha (EEF1A1 gene), prothymosin alpha, selenoprotein P (Sepp1), quinoid dihydropteridine reductase (QDPR) and cytochrome coxidase were down-regulated at E60 compared with those at E45 in the microarray. Validation of microarray data was achieved by using qRT-PCR. The six up-regulated and 6 down-regulated genes were changed accordingly (Figure 2C and 2D). (C) The six up-regulated genes were changed accordingly. (D) The six down-regulated genes were changed accordingly. Samples taken at E45 were used as controls. The relative levels of mRNA to GAPDH RNA were determined longitudinally by qRT-PCR assay. Data are expressed as mean relative values ± the standard error measurement (SEM) of each group of cells at each time point from three separate experiments. *P <0.05.