Figure 5
From: Mechanisms underlying dual effects of serotonin during development of Helisoma trivolvis(Mollusca)
Expression of the 5-HT 1 and 5-HT 7 receptors is differently regulated during H. trivolvis development. Relative changes in the amount of mRNA encoding for the 5-HT1 and 5-HT7 receptors were determined at different developmental stages, including trochophore (st 19), shortly before metamorphosis (st 23) and in the middle of metamorphosis (st 25) using real time quantitative RT-PCR and ΔΔCt method. (A) The 5-HT1R is transiently unregulated at stage 23 only. (B) Relative expression of 5-НТ7R gradually down regulated by the metamorphic stage (st 25).