Table 4 Sequence of the specific primers used for cloning and qPCR gene expression analysis
| Â | Cloning | For quantitative PCR | ||
|---|---|---|---|---|
Gene | Primer | Sequence (5′ → 3′) | Primer | Sequence (5′ → 3′) |
Pepsinogen A2 | ||||
| Â | PepA2-F | ATGAAGTGGCTCGTTGTTCTCT | PepA2-qF | TACGATGCCAACCACTTCA |
| Â | PepA2-R | TTACACGGACTTGGCCAGACCAATG | PepA2-qR | GATGGGCCAGCGATCAGGGAG |
H + /K + -ATPase α subunit | ||||
| Â | HKA-F | GTCTGGACTGTGCTTTGCT | HKA-qF | AGCCAATGTTGGCATCATCTCA |
| Â | HKA-R | CGCACAACAGCGGGAACCAG | HKA-qR | CGTCATCCAACTCCTCACT |
H + /K + -ATPase β subunit | ||||
| Â | HKB-F | ATGGCCGCCTTGAAGGAGAA | HKB-qF | GGAGAAGAGGACCTGTGG |
| Â | HKB-R | TTATTTCACTGCTTTCAGGGAA | HKB-qR | AGAACGCCAAGTAATACAA |
Ghrelin | ||||
| Â | Ghr-F | TTAACACTCTATGTCCCTTCATCA | Ghr-qF | GGCTGCTGGTTGTTCTACTCTG |
| Â | Ghr-R | GTCAGTTGATGCTTTATTTTTACCACC | Ghr-qR | TCCTCGGTGGGTTGATTCTG |
Na + /K + -ATPase α subunit | ||||
| Â | NaKA-F | CTGAAGGCAACGAGACTGT | NaKA-qF | CTGAAGGCAACGAGACTGT |
| Â | NaKA-R | GGATGACGAAATATGTGAAGAA | NaKA-qR | CGAGGTTCTGGCGAAGACGAT |
Elongation factor 1 alpha | ||||
| Â | EF-qF | CGAGAAGTTCGAGAAGGAAGCT | ||
EF-qR | ACCCAGGCGTACTTGAAGGA | |||