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Figure 2 | BMC Developmental Biology

Figure 2

From: Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A

Figure 2

Expression of heart development marker genes in chicken heart forming cell culture. Primary heart forming region (HFR) cells from 3–5 ss chicken embryos were cultured in DMEM and Endo-Grow medium and analyzed for VEGF-A, GATA4 and Nkx2.5 mRNA after 3 d in culture. 30 cycles of PCR amplification were run with cDNA isolated from 150,000 HFR cells. β-actin was used as a reference.

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BMC Developmental Biology

ISSN: 1471-213X

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