Figure 7

Modified FISH protocol for determining expression patterns of problematic genes. (A) Maximum intensity projection showing enriched expression of Smed-hb (magenta) in ciliated protonephridia labeled by anti-acetylated α-Tubulin (blue) and Smed-POU2/3 (green). A subset of POU2/3 positive putative protonephridial progenitor cells in close association with ciliated protonephridia coexpress hb (arrow). (B) Single confocal section showing coexpression of Smed-CHD4 (magenta) and smedwi-1 (green) in intact planarians stained with DAPI (blue) and the chromatoid body marker, Y12 (yellow). Most chromatoid bodies lack CHD4 mRNA (closed arrows). However, there are occasional chromatoid bodies in close association with CHD4 puncta (open arrowhead). (C) Maximum intensity projection of a cell expressing Smed-nog1 (magenta) and Smed-nog2 (green). Scale bar: 10 μm.