Table 2 RACE primers
From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
Name | 5′ or 3′ | Sequence (5′ → 3′) | No. of bases | Exons hitting | Position on NM_021099 |
|---|---|---|---|---|---|
e11 5′ | 5′ | CAGCCTGTTTCTGGG AAACTCCCATTTG | 27 | Exon 11 | 1825-1798 |
e12 5′ | 5′ | GCAACTGTCATGGC AGCATCCGACTT | 26 | Exon 12 | 1920-1895 |
e18 5′ | 5′ | TGCTCTCTGGTGCCA TCCACTTCAC | 25 | Exon 18 | 2552-2756 |
e20 5′A | 5′ | GGTCAGCGTCCCAG CAAGTCTTCAT | 25 | Exon 20 | 2786-2762 |
e20 5′B | 5′ | AAGGGGTCAGCGTC CCAGCAAGTCT | 25 | Exon 20 | 2790-2766 |
e20 5′C | 5′ | TGCTTGGTGCTGTCC GAGATCTGCT | 25 | Exon 20 | 2856-2832 |
e21 5′ | 5′ | GGGGTTGCAGTTTG CCAAGTTGGAG | 25 | Exon 21 | 2887-2863 |
e10 3′ | 3′ | AAATCCAGGCCCAC ACTCTGTTCACG | 26 | Exon 10 | 1602-1627 |
e11 3′ | 3′ | TGGGAGTTTCCCAG AAACAGGCTGAG | 26 | Exon 11 | 1802-1827 |
e18 3′ | 3′ | CCGTGAAGTGGATG GCACCAGAGAG | 25 | Exon 18 | 2550-2574 |
e19 3′A | 3′ | AGGAAGCAGCCCCT ACCCAGGGATG | 25 | Exon 19 | 2650-2674 |
e19 3′B | 3′ | GGGATGCCGGTCGA CTCCAAGTTCT | 25 | Exon 19 | 2669-2693 |
e20 3′A | 3′ | TGACCCCTTGAAAA GGCCAACATTCA | 26 | Exon 20 | 2782-2807 |
e20 3′B | 3′ | GCAGATCTCGGACA GCACCAAGCAC | 25 | Exon 20 | 2833-2857 |