Figure 9
From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
Expression of c-kit and other germ cell differentiation-related genes in the 5 dpp, 10 dpp and 60 dpp mouse testes stimulated by RA. 5 dpp, 10 dpp and 60 dpp testes were treated with 2 μM RA diluted in ethanol for 24 h in vitro. Testes treated with the same concentration of ethanol without RA were the control group. Realtime PCR was carried out for quantitative determination of the expression of c-kit and its potential regulatory genes (BMP4, Cyp26b1, DAZL, EGR3, Kitl, RARα and Stra8). Values of the vertical axis represented the expression fold change comparing with the control group. The results were normalized to GAPDH values. Error bars represent the S.E.M. Values with ** represented a significance with a P<0.01 whereas values with * represented a significance with a P<0.05 comparing with the control group. (A) c-kit expression in the 5 dpp testes. (B) Expression of germ cell differentiation-related genes in the 5 dpp testes. (C) c-kit expression in the 10 dpp testes. (D) Expression of germ cell differentiation-related genes in the 10 dpp testes. (E) c-kit expression in the 60 dpp testes. (F) Expression of germ cell differentiation-related genes in the 60 dpp testes.