Figure 8
From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
Immunofluorescence study of Kit protein expression in 5 dpp, 10 dpp and 60 dpp mouse testes. Images were obtained with ZEISS Axioplan 2 imaging system and Spot 4.7/SpotAdvanced software (Magnification × 400). Kit positive cells were stained with green or red fluorescein (Arrow). Leydig cells (Asterisk) displayed a large prominent nucleus. The cell nuclei was counterstained with 4′,6-diamidino-2-phenylindole. Images were merged with Photoshop CS4. (A) FITC labeled anti-Kit extracellular domain in 5 dpp mouse testes. White arrow: Kit positive cells (B) FITC labeled anti-Kit extracellular domain in 10 dpp mouse testes. (C) FITC labeled anti-Kit extracellular domain in 60 dpp mouse testes. (D) Alexa Fluo 594 labeled anti-Kit intracellular domain in 5 dpp mouse testes. (E) Alexa Fluo 594 labeled anti-Kit intracellular domain in 10 dpp mouse testes. (F) Alexa Fluo 594 labeled anti-Kit intracellular domain in 60 dpp mouse testes.