Figure 4

Profiles of c-kit transcripts in 5 dpp, 10 dpp and 60 dpp testes. (A) Relative c-kit mRNA expression level in the 5 dpp, 10 dpp and 60 dpp mouse testis. Error bars represent S.E.M. c-kit e7-8, real-time PCR using probe hit full-length c-kit transcripts on exons 7-8. c-kit e20-21, real-time PCR using probe hit full-length c-kit transcripts on exons 20-21. c-kit e21, real-time PCR using probe hit full-length c-kit transcripts on the end of exons 21. Differences between each two groups were significant (P<0.05). (B) The ratio of Full-length and truncated c-kit transcripts in 5 dpp, 10 dpp and 60 dpp mouse testes. The ratio of truncated c-kit transcripts were calculated by the formula (1-ratio_{full-length transcripts}) × 100%. (C) The ratio of long 3′ UTR and short 3′ UTR c-kit transcripts in 5 dpp, 10 dpp and 60 dpp mouse testes. The ratio of short 3′ UTR c-kit transcripts were calculated by the formula (1-ratio_{long 3′UTR transcripts}) × 100%.