Figure 2

RACE PCR products gel electrophoresis. (A) 5′ and 3′ RACE with primer sets hit exons 10-12. c18-4 had two 5’ ends (1.8 kb and 1.4 kb). CRL-2053 and 60 dpp testes had one 5’ end (1.8 kb). c18-4 had one 3 kb 3’ end (3 kb). CRL-2053 and 60 dpp testes had two 3’ end (3 kb and 1.8 kb). (B) 5′ and 3′ RACE with primer sets hit exons 10-12. 5 dpp and 10 dpp testis had one 5’ ends (2 kb). 5 dpp and 10 dpp testes had two 3’ end (3 kb and 2.1 kb). CRL-2053 and 60 dpp testes had two 3’ end (3 kb and 1.8 kb). (C) 5′ and 3′ RACE with primer sets hit exons 18-21. c18-4 had two 5’ ends (2.9 kb and 1.5 kb). CRL-2053 had three 5’ ends (2.9 kb, 1.4 kb and 1.6 kb). 5 dpp, 10 dpp and 60 dpp testes had the same one 5’ end (2.9 kb); c18-4 had one 3’ end (2.9 kb). CRL-2053 5 dpp and 10 dpp and 60 dpp testes had two 3’ end (3 kb and 1.8 kb). (D) Summary of c-kit RACE PCR fragments from cell lines and testis.