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Figure 1 | BMC Developmental Biology

Figure 1

From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation

Figure 1

c-kit northern hybridization. (A) RNA electrophoresis. 10 μg total RNA from different samples were loaded. RNA sizes were marked with 2 μg (2 μl) RNA Millennium size markers (Ambion). All RNA samples were in good qualities. (B) Northern hybridization with c-kit probe hit exons 10-12. Short transcripts (purple arrowhead) with a size between 4 and 5 kb could be observed in c18-4, CRL-2053 and different aged testes. Long transcripts (blue arrowhead) with a size between 5 and 6 kb were seen in 10 dpp and 60 dpp testes. (C) Northern hybridization with c-kit probe hit exons 18-20. A 1.5 kb short transcript (red arrowhead) was observed in c18-4 and a 2.7 kb short transcript (green arrowhead) was observed in CRL-2053. Short transcripts (purple arrowhead) with a size between 4 and 5 kb could be observed in 5 dpp, 10 dpp and 60 dpp testes. Long transcripts (blue arrowhead) with a size between 5 and 6 kb were seen in 10 dpp and 60 dpp testes. (D) Northern hybridization of beta-actin (internal control).

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