Figure 2
From: The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
Ablation of the lateral lip of the dermomyotome after GFP labelling. A and B are scheme of the operation. A: The lateral part of interlimb somites was electroporated with a GFP vector. B: After one day reincubation, a GFP marked lateral lip was removed. C-F are pictures of a same embryo. C: One day after the electroporation. The lateral parts of 5 somites were labelled with GFP. D: Immediately after the removal of the most lateral part of a somite (arrow). E: One day after the ablation. GFP domain of the ablated dermomyotomal lip did not grow as far ventrally as the unoperated somites. F: The Pax3-expression was affected in the operated segment (arrows). Abbreviations: m medial, l lateral.