Developmental | 5-cell (n) | 8-16 cell (n) | Morula (n) | Blastocyst | Blastocyst | Hatched |
---|---|---|---|---|---|---|
Stage | Â | Â | Â | 7dpi (n) | 8/9dpi (n) | Blastocyst |
 |  |  |  |  |  | 9dpi (n) |
Number of immunostained embryos per staining set- up (n) | Â | Â | Â | 1 (5) | Â | 1 (5) |
 |  | 1 (4) | 2 (4) |  | 2 (5) | |
1 (10) | 1 (5) | 2 (4) | 3 (4) | Â | 3 (6) | |
2 (10) | 2 (4) | 3 (4) | 4 (4) | Â | 4 (5) | |
3 (10) | 3 (4) | 4 (4) | 5 (4) | 6 (5) | 5 (6) | |
4 (10) | 4 (4) | 6 (6) | 6 (9) | 7 (5) | 6 (12) | |
5 (10) | 5 (5) | 8 (4) | 7 (8) | 10 (10) | 7 (11) | |
 | 9 (20) | 9 (11) | 8 (6) |  | 8 (13) | |
 |  | 10 (5) | 9 (4) |  | 9 (10) | |
 |  |  | 10 (8) |  | 10 (12) | |
Antibody staining set- up | 1 – CDX 2 (ab15258) mouse | 6 – CDX 2 (ab15258) mouse + OCT4 (ab18976) rabbit | ||||
 | 2 – CDX 2 (ab88129) rabbit | 7 – CDX 2 (ab88129) rabbit + OCT4 (ab27985) goat | ||||
 | 3 – OCT4 (ab27985) goat | 8 – CDX 2 (ab15258) mouse + NANOG (500-P236) rabbit | ||||
 | 4 – OCT4 (ab18976) rabbit | 9 – CDX 2 (ab15258) mouse + H3K9me3 (07–442) rabbit | ||||
 | 5 – NANOG (500-P236) rabbit | 10 – OCT4 (ab27985) goat + NANOG (500-P236) rabbit |