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Table 2 Experimental groups and antibody set-up for single and double immunolabelling

From: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development

Developmental

5-cell (n)

8-16 cell (n)

Morula (n)

Blastocyst

Blastocyst

Hatched

Stage

   

7dpi (n)

8/9dpi (n)

Blastocyst

      

9dpi (n)

Number of immunostained embryos per staining set- up (n)

   

1 (5)

 

1 (5)

  

1 (4)

2 (4)

 

2 (5)

1 (10)

1 (5)

2 (4)

3 (4)

 

3 (6)

2 (10)

2 (4)

3 (4)

4 (4)

 

4 (5)

3 (10)

3 (4)

4 (4)

5 (4)

6 (5)

5 (6)

4 (10)

4 (4)

6 (6)

6 (9)

7 (5)

6 (12)

5 (10)

5 (5)

8 (4)

7 (8)

10 (10)

7 (11)

 

9 (20)

9 (11)

8 (6)

 

8 (13)

  

10 (5)

9 (4)

 

9 (10)

   

10 (8)

 

10 (12)

Antibody staining set- up

1 – CDX 2 (ab15258) mouse

6 – CDX 2 (ab15258) mouse + OCT4 (ab18976) rabbit

 

2 – CDX 2 (ab88129) rabbit

7 – CDX 2 (ab88129) rabbit + OCT4 (ab27985) goat

 

3 – OCT4 (ab27985) goat

8 – CDX 2 (ab15258) mouse + NANOG (500-P236) rabbit

 

4 – OCT4 (ab18976) rabbit

9 – CDX 2 (ab15258) mouse + H3K9me3 (07–442) rabbit

 

5 – NANOG (500-P236) rabbit

10 – OCT4 (ab27985) goat + NANOG (500-P236) rabbit