Skip to main content

Table 1 Primer pairs and annealing conditions used for the real-time PCR gene expression analyses

From: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development

Name

Sense primer

Antisense primer

Product size

Annealing temperature

CDX2

CTTTCCTCCGGATGGTGATA

AGCCAAGTGAAAACCAGGAC

113 bp

58°C

c-MYC

TGGACGCTAGATTTCCTTCG

GCTGCTGCTGGTGGTAGAAG

155 bp

58°C

FN1

GCACTATGGCCAAAGAGAGG

AACAGGAGACAAGGGTGTGG

109 bp

58°C

KLF4

AAACCAAAGAGGGGAAGACG

ATGTGTAAGGCGAGGTGGTC

291 bp

58°C

KRT18

GAGATCGAGGCTCTCAAGGA

GTCCAGCTCCTCTCGGTTCT

201 bp

58°C

NANOG

AAACAACTGGCCGAGGAATA

AGGAGTGGTTGCTCCAAGAC

194 bp

58°C

OCT4

GTTTTGAGGCTTTGCAGCTC

CTCCAGGTTGCCTCTCACTC

185 bp

58°C

GATA6

CTGCGGTCTCTACAGCAAGA

GTGGTCGTGGTGTGACAGTT

117 bp

58°C

REX1

GGAAGAGGACCCACTCCTTC

ACTTGGCCTCCTAGTGCATC

241 bp

58°C

18S rRNA

GGAGAGGGAGCCTGAGAAAC

TCGCGGAA GGATTTAAAGTG

170 bp

58°C