Figure 4
From: The role of Zic transcription factors in regulating hindbrain retinoic acid signaling
Retinoic acid-responsive gene expression in Zic morpholino-injected embryos. mRNA transcript expression of RA-dependent markers meis3 and otx2 (A, B), hnf1ba (C, D), hoxd4a (E, F), and hoxb1a (G, H) were compared in control uninjected (A, C, E, G) and Zic2a;Zic2b;Zic3MO-injected embryos (B, D, F, H). Although hoxb1a and hnf1ba are largely unchanged, a decrease in meis3 and hoxd4a is observed in Zic morpholino-injected embryos. All images are of 10.5 hpf embryos in dorsal views with anterior oriented to top.