Figure 3
From: The role of Zic transcription factors in regulating hindbrain retinoic acid signaling
Zic2a and Zic2b depletion causes down-regulation of the retinoid-metabolism genes, cyp26a1 and aldh1a2 , during early embryogenesis. mRNA in situ hybridization analysis of cyp26a1 (A, B) and aldh1a2 (C, D, F-I) expression reveals a reduction in both genes in Zic2a;Zic2b;p53 morpholino injected embryos (B, D, G, I) compared to control p53-morpholino injected embryos (A, C, F, H). Relative aldh1a2 mRNA expression levels for 7 hpf embryos were determined using quantitative real-time PCR (E). Levels reflect average of 7 technical replicates, with aldh1a2 mRNA at 0.71 significantly reduced (SD = 0.11; *p < 0.0001) compared to normalized control. Images are of 7 hpf embryos in dorsal view with anterior oriented to top (A-D), or lateral views with anterior to the left of 18 hpf (F, G) or 24 hpf embryos (H, I). Error bars depict standard deviation.