Table 2 Insulin-induced time-dependent changes in intact denuded Rana pipiens Oocytes 1
Additions | Fluid phase exchange | Membrane potential | Membrane conductance | 2-Deoxy-glucose uptake | pHi | Na+ | |
|---|---|---|---|---|---|---|---|
|  | nl/h/oocyte | mV | μS | nmols/h/oocyte |  | Electrode2 | |
None | +Na+ | 23.4 ± 1.23 (5) | −42.6 ± 1.6 (8) | 5.2 ± 0.3 (14) | 1.05 ± 0.19(3) | 7.38 ± 0.035 (7) | 44.2 ± 2.4 (3) |
|  | -Na+ | 7.68 ± 1.8 (3) | −75.0 ± 4.2 (4) |  | 0.12 ± 0.07 (3) | 7.24 ± 0.040 (3) |  |
Insulin, 10 μM | +Na+ | 65.5 ± 2.4 (3) | −59.2 ± 1.7 (6) | 1.8 ± 0.4 (4) | 3.05 ± 0.29 (3) | 7.65 ± 0.017 (3) | 19.0 ± 2.0 (3) |
|  | -Na+ | 7.94 ± 0.65 (3) | −69.0 ± 3.2 (4) |  | 0.17 ± 0.06 (3) | 7.26 ± 0.021 (3) |  |
Progesterone +Na+,1.65 μM | 18.8 ± 0.29 (3) | −52.0 ± 4.5 (4) | 6.4 ± 0.5 (8) | 1.03 ± 0.16 (3) | 7.69 ± 0.34 (4) | ........ | |
Insulin, 10 μM + Na+ +PMSF, 1.0 mM3 | 13.8 ± 1.7 (3) | −38.0 ± 1.5 (6) | 5.0 ± 0.4 (4) | 1.11 ± 0.23 (3) | 7.24 ± 0.26 (3) | ........ | |