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Figure 5 | BMC Developmental Biology

Figure 5

From: Plasma membrane events associated with the meiotic divisions in the amphibian oocyte: insights into the evolution of insulin transduction systems and cell signaling

Figure 5

Effect of 5 μM insulin on serine protease activity in isolated, intact plasma-vitelline membranes from prophase-arrested Rana pipiens oocytes (● - ●). p-Tosyl-l-arginine methyl ester (TAME) was used as a serine protease-specific substrate and the serine protease inhibitor phenylmethyl-sulfonyl-flouride (PMSF) was added 15 min prior to insulin (▲ - ▲). The control is represented by (○ - ○). Values shown are from a typical experiment using sibling oocytes. The mean ± SD values for serine protease activity for oocytes from four R. pipiens females at 30 min was 26.4 ± 2.57 absorbancy units for 5 μM insulin, 8.23 ± 1.27 absorbancy units for 0 insulin (controls) and 0 absorbancy units for oocytes treated with both 5 μM insulin and PMSF. For details see methods.

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BMC Developmental Biology

ISSN: 1471-213X

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