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Figure 2 | BMC Developmental Biology

Figure 2

From: Plasma membrane events associated with the meiotic divisions in the amphibian oocyte: insights into the evolution of insulin transduction systems and cell signaling

Figure 2

Scatchard type plot of [125I]insulin binding to the plasma-vitelline membrane complex of denuded R. pipiens oocytes. Intact, denuded prophase oocytes were incubated in Ringer’s solution containing [125I]insulin for 15 min at 20–22°C, the oocytes removed, rinsed with Ringer’s solution and the membranes removed and counted as described in Methods. The [125I]insulin binding data were corrected by subtracting the constant binding fraction.

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