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Figure 2 | BMC Developmental Biology

Figure 2

From: The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophilawing margin in a cyclin B-dependent manner

Figure 2

EcR is required for normal patterning of E2F1 activity in the wing margin. (A-D) - PCNA-GFP/+ wing imaginal discs stained with the EcR antibody. PCNA-GFP (A) is normally detected in cycling cells of the wing pouch and in the G1 band within the anterior and posterior of the wing margin. EcR staining in red (B) overlaps with PCNA-GFP throughout the pouch and the G1 cells of the margin (C and D, DNA in blue). (E-H) - EcR RNAi flip out clones generated in the PCNA-GFP background are marked with CD8-RFP. White arrows mark ectopic G1 cells in the G2 region. The DNA stain in H is for the equivalent confocal section and white arrows show the position of ectopic PCNA-GFP corresponding to clones in the anterior, while the red arrow corresponds to the clone with reduced PCNA-GFP in the posterior.

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BMC Developmental Biology

ISSN: 1471-213X

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