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Figure 5 | BMC Developmental Biology

Figure 5

From: Tc-knirps plays different roles in the specification of antennal and mandibular parasegment boundaries and is regulated by a pair-rule gene in the beetle Tribolium castaneum

Figure 5

Expression of Tc-ems and Tc-slp-1 in wildtype, Tc-kni RNAi and antennaless embryos. Panels B, D, E, G and H represent documentation of fluorescent FastRed stainings of the respective gene and are therefore shown in grayscale. Tc-ems expression in the antennal (white arrowhead, panels A, B) and mandibular (black arrow, panels A, B) segments is abnormally fused in Tc-kni RNAi embryos (red arrows in panel B; compare to panel A). A black arrowhead marks the maxillary domain of Tc-ems expression in panels A, B. The antennal Tc-slp-1 expression domain (white arrowhead, panels C-H) is often expanded, while the mandibular (black arrow, panels C-H) and maxillary (black arrowhead, panels C-H) Tc-slp-1 expression domains are fused (red arrows in panels D-E, G-H) in Tc-kni RNAi and antennaless embryos. Ventral views, and anterior to the top, in all panels.

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