Figure 4

Expression of Tc-gsc and Tc-lim1 in wildtype and Tc-kni RNAi embryos. All embryos are also co-stained for Tc-wg. Tc-gsc expression is reduced in posterior-lateral regions of its normally wedge-shaped ocular/antennal expression domain in early germband Tc-kni RNAi embryos (black arrows in panels B, D; compare to panels A, C respectively). At slightly later germband stages however, clear differences between the Tc-gsc expression domain in Tc-kni RNAi and wildtype embryos are no longer observed (compare panel F to panel E). Tc-lim1 expression is also irregular and reduced in posterior regions of its wedge-shaped ocular/antennal expression domain in early germband Tc-kni RNAi embryos (black arrowheads in panel H; compare to panel G). At slightly older germband stages clear differences in the Tc-lim1 expression domain are no longer observed between Tc-kni RNAi and wildtype embryos (compre panel J to panel I). In later wildtype germband embryos, the Tc-lim1 ocular/antennal domain splits into two stripes (marked by white arrowheads in panel K). This fails to occur in equivalent stage Tc-kni RNAi embryos, and antennal, mandibular and maxillary expression domains are often fused in lateral regions of the germband (red arrowheads in panel L; compare to panel K). Ventral views, and anterior to the top, in all panels.