The C. elegans gene pan-1encodes novel transmembrane and cytoplasmic leucine-rich repeat proteins and promotes molting and the larva to adult transition

Table 1 pan-1(RNAi) interactions with heterochronic genes

Genotype	RNAi (N)	% With alae	% Arrested gonadal growth¹	% Arrested vulva development²
WT	GFP (10)	100	0	0
	pan-1 (31)	0	88.7	100
lin-28 (n719)	GFP (15)	100	0	0
	pan-1 (40)	5.0	100	100
hbl-1(ve18)	GFP (15)	100	0	0
	pan-1 (38)	7.9	100	89.5³
lin-29 (n333)	GFP (15)	0	0	0
	pan-1 (41)	0	85.4	14.6³
let-7(n2853)	GFP ⁴	NA	NA	NA
	pan-1 (30)	0	96.7	93.3

At least two independent RNAi experiments were scored and numbers combined. Experiments were performed at 20°C and phenotypes were scored 54 + hrs post-feeding, except for let-7(n2853) which was performed at 25°C and scored at 72 hrs post-feeding.
¹Defined as gonad arms that failed to reflex. The gonad arms of hermaphrodites were scored independently (i.e. two gonad data points/animal).
²Defined as a vulva that invaginated but did not evert.
³Animals that had pronounced Pvls were not examined (see text). The animals that did not have arrested vulva development had small protruding vulvas (Figure 7B).
⁴These animals had “exploded out of the vulva” at the scoring time point and were not observed at high magnification (see Figure 7).
NA – not applicable.

ISSN: 1471-213X