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Figure 6 | BMC Developmental Biology

Figure 6

From: The C. elegans gene pan-1encodes novel transmembrane and cytoplasmic leucine-rich repeat proteins and promotes molting and the larva to adult transition

Figure 6

pan-1::GFP expression pattern. (A) Expression of pan-1prom::GFP in the somatic gonad and vulva. The proximal sheath (Sh), spermatheca (Sp), and uterus (Ut) and vulva are indicated. (B) pan-1prom::GFP expression in body wall muscle. Arrow indicates muscle cell nucleus. (C) A merged epiflourescence and DIC micrograph showing expression of PAN-1FL::GFP in the spermatheca, uterus, and vulva. The arrows indicate PAN-1FL::GFP punctae in the apical (lumenal surface) regions of spermatheca and uterine epithelia. (D) A ventral view of a developing L4 stage vulva showing PAN-1FL::GFP punctae clustered on the apical ends of vulva epithelial cells. Cytoplasmic fluorescence is also observed in these cells (E) PAN-1FL::GFP in a seam cell. Arrow indicates a punctate region of localization and cytoplasmic fluorescence is also observed. (F) Expression of PAN-1FL::GFP in the pharynx and anterior hypodermis. Cytoplasmic fluorescence is also observed in the pharyngeal tissue. Arrow indicates punctate localization in the hypodermis. Immunolocalization of MH27 (G) and PAN-1FL::GFP (H) in the developing pharyngeal epithelium. PAN-1FL::GFP is found in the developing pharyngeal epithelium (arrow) of a bean stage embryo. (I) Merged image of (G) and (H). Scale bars = 10 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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