Figure 3

Drosophila PRL is localized on the plasma membrane of imaginal disc cells and enriched in developing axons of pupal retina. (A-F) Expression of Drosophila PRL in larval eye imaginal discs. Eye antennal discs of 3^rd instar larvae were dissected and stained with anti-dmPRL antibody. DmPRL was expressed all over the eye discs but is more enriched in differentiating retinal cells (A). The arrow indicates the axon bundles of photoreceptor cells. In panel (A-C), the indicated insets are reproduced at (D-F) to show the enlarged view of the same field. DmPRL (D) was partially colocalized with phalloidin (E) in the differentiating eye disc cells (F). (C and F) Merged images. (G-I) DmPRL expression on developing pupal eye discs. In pupal discs at 25% pupal development, DmPRL was found to be enriched in developing axon (G). The indicated inset of (G) is reproduced at (H) to show the enlarged view of dmPRL staining. In pupal discs at 50% pupal development, dmPRL can be detected in the plasma membrane of developing retinal cells (I). (J-O) Expression of Drosophila PRL in larval wing imaginal discs. DmPRL was ubiquitously expressed throughout the wing discs (J). In panel (M-O), the indicated insets was reproduced at (J-L) to show the enlarged view of the same field. DmPRL (J) was colocalized with phalloidin (K). (L and O) Merged images. (P-U) Expression of Drosophila PRL in larval leg imaginal discs. DmPRL was ubiquitously expressed throughout the leg discs (P). In panel (S-U), the indicated insets was reproduced at (P-R) to show the enlarged view of the same field. DmPRL (S) was colocalized with phalloidin (T). (R and U) Merged images.