Figure 3

In vivo infection of regenerating limbs with foamy versus lentivirus vector particles. Concentrated FV or LV vector particles were injected into 5-day limb blastemas, which were then allowed to regenerate. Images were taken 28 days post-injection. A. FV vector particle (EGFP) injected limb. B. FV vector particle injected limb that had regenerated, and then was reamputated and regenerated a second time showing stability of expression. C. FV vector particle (DsRed) injected limb. D. FV vector particle (DsRed) injected tail. Asterisk point to labeled blood vessels. The inset shows high magnification of the fin where blood vessels are labeled. E, F. LV vector particle (EGFP) injected limb in bright field (E), and fluorescence (F) shows no fluorescence signal. Scale Bars: A,C: 500 μm B,D,E,F: 1 mm.