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Figure 1 | BMC Developmental Biology

Figure 1

From: Foamy virus for efficient gene transfer in regeneration studies

Figure 1

Foamy virus displays high efficiency of transduction with stable expression in cultured newt cells. A+B) Schematic outline of the FV (A) and lentiviral (B) vector systems used. FV or LV components are indicated in grey, heterologous transcriptional control elements in black, and the transgene and posttranslational control element in white. C) The Newt myogenic cell line A1, was transduced with FV (FV/FV, solid squares) or LV (HIV/VSV-G, solid circles) vector particles expressing a hUbiC promoter-driven EGFP transgene at a MOI of 130. In weekly intervals the percentage of EGFP expressing cells was determined by flow cytometry. The initial rate of expression was similar for both viruses but FV transduced cells increased over time, plateauing at 3 weeks post-infection at approximately 75% expressing cells. In contrast, a large number of LV transduced cells lost expression after initial infection and plateaued at 20% expressing cells. PFV based transfer vector (PV) and packaging constructs for PFV glycoprotein (PE, ENV), PFV polymerase (PP, POL) and PFV capsid (PG, GAG); HIV-1 based transfer vector (LV) and packaging constructs for HIV-1 capsid (GAG) and polymerase (POL) (LG/P) and VSV glycoprotein (VG, VSV-G). CMV: cytomegalo virus immediate early enhancer – promoter; R: long terminal repeat (LTR) repeat region; U5: LTR unique 5’ region; ΔU3: enhancer-promoter deleted LTR unique 3’ region; CASI+II: FV cis-acting sequences I and II; hUbiC: human ubiqutin C promoter; WPRE: Woodchuck hepatitis virus posttranscriptional regulatory element; : HIV-1 packaging sequence; RRE: rev-responsive-element; cPPT: central poly purine tract; SD: splice donor; SA: splice acceptor.

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