Figure 3
From: Expression pattern of Protein Kinase C ε during mouse embryogenesis
PKCϵ expression is highest within the nervous system at E13.5. A, at embryonic stage E13.5, whole mount staining allowed for the detection of LacZ signal in choroid plexus (cho), ventral part of the medulla oblongata (vmo) and pinna of the ear (pin). B-H, 15 μm sagittal sections at this stage showed incomplete penetration of X-Gal at previously reported domains such as dorsal root ganglia (drg) and trigeminal (V) ganglion (trg). Lac Z signal at this stage was also detected in neural tube (nt), urogenital sinus (uro), caudal part of lower jaw (jaw), heart (hea), mucosal lining of the stomach (sto), and limbs (lim). I, Immunohistochemistry at E13.5 included staining of PKCϵ deficient sections as negative control. J, sagittal sections confirmed ganglia as the domain with highest expression of PKCϵ at this stage. K-P, Immunostaining of cross sections (K-N) and sagittal sections (O and P) confirmed PKCϵ detected via LacZ staining in trigeminal ganglion (trg), choroid plexus (cho), neural tube (nt), urogenital sinus (uro), mucosal lining of the stomach (sto), kidney (kid), heart, caudal part of lower jaw (jaw) and limbs (lim). In addition, it also allowed for detection of PKCϵ in hypothalamus (K, hyp).