Figure 6

Expression of CBF:H2B-Venus reporter at E8.5-E9.5 with specific localization in endothelial cells. Expression of CBF:H2B-Venus in E8.5 embryos (A-C). Lateral whole mount view of an E8.5 embryo (A). Transverse sections through the anterior and posterior regions of E8.5 embryo depicting reporter expression in dorsal aorta (da) (B-C) as well as intermediate mesoderm (red arrowhead, B1). Expression of CBF:H2B-Venus at E9.5 (D-J). Lateral views of same E9.5 embryo to highlight the plane of section (D, D1, D2, D3). Transverse sections through anterior and posterior regions of E9.5 embryo depicting reporter expression in da (E, E1, E2, G, G1, G2), head vessels (E, red arrow in E1, E2, J, red arrow in J1 and J2), umbilical veins (G, G1, G2), intersomitic vessels (isv, white arrow in H, H1 and H2), pharyngeal arch arteries (yellow arrow in I, I1 and I2) and mesoderm of the pharyngeal arches (pa, I, I1, I2 and I3). Expression of CBF:H2B-Venus counterstained with Hoechst to label nuclei in E8.5 embryo (B, C), in E9.5 embryo (E, F, G, H, I, J). Expression of CBF:H2B-Venus in E8.5 (B1, C1) and E9.5 (E1, F1, G1, H1, I1, J1) embryos. Expression of CBF:H2B-Venus counterstained with phalloidin to label F-Actin in E8.5 (B2, C2) and E9.5 (E2, F2, G2, H2, I2, J2). Co-staining with PECAM (K-O and O1), a marker for endothelial cells in E9.5 reveals localization of CBF:H2B-Venus reporter expression to endothelial cells in the pharyngeal arches (K), dorsal aorta (L), head vessels (L), umbilical veins (M), tail bud (N), intersomitic vessels (O, white arrow) and limb bud (O). IV, fourth ventricle; hg, hindgut; lb, limb bud; np, neural plate; nt, neural tube; ov, otic vesicle; ppc, pericardio-peritoneal canal; som, somites; uv, umbilical veins.