Figure 5

Expression of CBF:H2B-Venus at fetal stages (~E14.5). Stereo widefield image of a lateral view of an E14.5 embryo expressing CBF:H2B-Venus (A). Transverse sections of E14.5 embryo showing CBF:H2B-Venus reporter expression (B1, C1, D1, E1, F1, G1, H1, I1) and CBF:H2B-Venus expression and Hoechst to label nuclei, respectively (B, C, D, E, F, G, H, I). CBF:H2B-Venus expression at E14.5 in the lens (l) and the pigmented part of retina (pr) (B,B1). Red arrowhead points to expression in cuboidal epithelium in anterior part of the lens. Red arrow points to Notch expression in cells of equatorial region of the lens. Expression of CBF:H2B-Venus in the interstitial mesenchymal cells of the lung (C, C1), in cells of the forming valves of the heart (D, D1), the gut (E, E1), the liver (F, F1) as well as in the vasculature of the digits (G, G1), cells of the thymus (H, H1) and nasal process (I, I1). a, atrium; br, bronchus; c, cornea; nc, nasal cavity; nr, neural part of retina, v, ventricle.