Figure 1
From: A bright single-cell resolution live imaging reporter of Notch signaling in the mouse
CBF:H2B-Venus construct design and validation in MEF cells. CBF:H2B-Venus MEFs are derived from E13.5 transgenic embryos and transfected with a pCX:NICD construct. Ectopic expression of NICD activates the canonical Notch signaling pathway and in turn leads to transgene expression, which can be assessed using flow cytometry. FACS analysis shows transgenic CBF:H2B-Venus MEFs comprise a Venus negative and Venus positive population and display an increase in fluorescence upon ectopic NICD expression.