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Figure 6 | BMC Developmental Biology

Figure 6

From: Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation

Figure 6

Representative control eggs matured in vitro from injected oocytes, showing normal, bipolar meiotic spindles. (A-D) Images of an oocyte injected with 0.01 mM of the inverted 14-3-3η morpholino. (E-H) Images of an oocyte injected with 0.01 mM the 14-3-3γ morpholino. (I-L) Images of an oocyte injected with 10 pL of deionized water. In all cases these oocytes, representative of others (see Figure 4) both injected and uninjected, were held for 24 hours in prophase arrest, released from arrest for13 hours, fixed, permeabilized and immunolabeled for confocal immunofluorescence with an antibody to α-tubulin (green), the antibody to 14-3-3η protein (red), and counterstained with Hoechst 3342 (blue) to visualize DNA. The panel at the far right is the merged overlay of immunofluorescence images from all three channels. In all cases, the spindle appears normal and 14-3-3η accumulates at the spindle. PB, first polar body. MO, morpholino oligonucleotide. Scale bars represent 10 μm.

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