Figure 2

The 14-3-3η protein accumulates and co-localizes with α-tubulin in MI and MII meiotic spindles during oocyte maturation. Representative cells were fixed, permeabilized and immunolabeled for confocal microscopy during oocyte in vitro maturation at the times indicated. The left column shows the merged images of α-tubulin (green) and the counterstain Hoechst 33342 (blue) to visualize the DNA. The right column shows each corresponding image of the 14-3-3η protein (red). (A-H) Paired confocal images at a single confocal plane of the spindle region of representative cells at the times indicated. (I-L) Representative egg imaged at the plane of metaphase II spindle (I,J) and at a different confocal plane to show the first polar body (K,L) attached to the same egg cell. Scale bars represent 10 μm.