Figure 1

The 14-3-3η protein accumulates at the metaphase II spindle of the mouse egg matured in vivo and in vitro . Cells were fixed, permeabilized and immunolabeled for confocal double immunofluorescence using one of two primary antibodies against the 14-3-3η protein (red), an antibody to α-tubulin (green) and counterstained with Hoechst 33342 (blue) to visualize DNA. (A-D) A representative in vivo-matured and ovulated egg labeled with a goat antibody that recognizes the C-terminal end of the 14-3-3η protein (C). (E-H) A representative in vitro-matured egg cell that was held in prophase I arrest for 24 hours, released from the arrest and examined at 13 hours with a rabbit antibody recognizing the N-terminal end of the 14-3-3η protein (G). PB, First Polar Body. The merged image is an overlay of immunofluorescence images from the three channels. Scale bars represent 10 μm.