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Figure 5 | BMC Developmental Biology

Figure 5

From: Integrins are required for cardioblast polarisation in Drosophila

Figure 5

Integrins concentrate at the presumptive luminal domain. Transverse optical sections from abdominal segments 6-8 of immunolabeled embryos reveal that βPS1 Integrin (red) is concentrated ventral to the leading edge of migrating CBs (arrowhead, A, A'), where the lumen forms at stage 17 (arrowhead, B, B'). Dystroglycan (Dg, green) outlines most of the cell surface during migration, and concentrates at the lumen surface later than Integrin (A, B). However, Dystroglycan is excluded from the cell junction between contralateral CBs, which instead accumulates Discs-Large (Dlg, arrow, red) at stage 17 (C, C'). Zygotic scb2 mutants accumulate lower levels of βPS1 Integrin apically during migration (arrowhead, D, D'). Integrin and Dystroglycan are both located apically at stage 17, but lumen formation fails (arrowhead, E, E'). An adherent domain marked by continuous Dlg labeling (arrow, F, F') indicates that no lumen forms, although a pocket of apical Dystroglycan labeling is still seen (arrowhead, F). The dorsal leading process apparent in yw migrating CBs (arrow, A) is missing in scb2 mutants (arrow, D). Dorsal at top; asterisks label approximate location of CB nucleus. Scale bar is 5 microns

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