Figure 2

(a) Multilineage differentiation potential of hMSC-TERT, hADMSCs, hADSSCs and hNFSSCs. Cells were induced for 15 days under osteogenic conditions then were assessed for alkaline phosphatase activity (ALP) to measure osteogenesis, while cells induced for 15 days under adipogenic conditions were assessed by the development of neutral lipid vacuoles stainable with Oil Red O (shown at magnification of 10×). (b) Quantitative real-time PCR (qRT-PCR) analysis of adipocyte and osteoblast-associated gene expression in induced cells. After differentiation for 15 days, cells were harvested, and total RNA was extracted and the expression of adipocyte and osteoblast-associated genes were quantified. Gene expression was normalized to beta-actin and is presented as fold change compared to control (non-induced cells). Data are shown as mean ± S.D of at least two independent experiments, n = 5 (except hMSC-TERT cell line).