Figure 4

Strategy to establish the transcriptional profile of accessory cells combining FACS and microarrays. A. Schematic of the experimental procedures. B. illustration of the gating used for the FACS. The left plot (showing the P1 gate) was used to sort cells according to cell size (forward scatter, FSC-A) vs. granularity (side scatter, SSC-A). The right plot discriminated cells according to GFP fluorescence intensity (GFP FITC-A) vs. Phycoerythrin (PE-A). Gates were selected as shown to sort GFP negative (GFP Neg) and GPP positive (GFP Pos) cells.