Figure 7
From: Regulation of aggregate size and pattern by adenosine and caffeine in cellular slime molds
Caffeine alters cAMP signal relay by affecting cAMP phosphodiesterase synthesis (PdsA). A) Aggregation pattern of AX2, acaA- , pdiA- and pde4- cells in the presence or absence of either caffeine or adenosine. Caffeine rescued the pdiA- and pde4- cells into wild type pheonotype. B) Rescuing of acaA- null phenotype by mixing the AX2 cells with acaA- cells in 4: 1 and 3: 2 ratio and checking the aggregation in presence of either caffeine or adenosine. C) Western blot of PdsA protein, to check the expression levels in vegetative AX2 cells pulsed or unpulsed with cAMP and pde4- cells pulsed with cAMP.