Figure 2

Effect of adenosine and caffeine on aggregation pattern of Dictyostelium and Polysphondylium. A) Aggregation pattern of the Polysphondylium cells a) Control (b) with 0.5 mM (c) with 1 mM adenosine. Small aggregate formation in the presence of caffeine (d) 1.0 mM (e) 2.5 mM. Scale bar = 200 μm. Polysphondylium amobae were plated at a density of 1 × 10⁶ cells/cm² on KK2 plates containing caffeine or adenosine and were allowed to form aggregates in the dark and humid conditions. (B) Aggregate size (Student's t-test, *p < 0.001, **p < 0.004). (C) The number of aggregates (Student's t-test, *p < 0.001). There were few aggregation centres, in the presence of adenosine. Many, small aggregates were formed in the presence of caffeine. Numbers of aggregates were counted in 1 cm² area. All values represent the mean ± standard error (n = 10).