Figure 7

Pdcd4 interacts genetically with eIF4A and bam. (A-D, F-I) Immunofluorescence images showing single projections of confocal Z-stacks of germaria from 5-day-old females. Genotypes are listed in the bottom right corner of each panel. Hts is labeled in green and Vasa is labeled in red. Asterisks indicate the locations of apical tips of the germaria. Scale bars are 10 μm. (E, J) Frequency histograms showing the mean number of cells containing spherical spectrosomes ± standard error for the indicated genotypes. (E) The p-value shown corresponds to the comparison of the number of spectrosome-containing cells in Pdcd4¹/Pdcd4¹compared to Pdcd4¹/Pdcd4¹; eIF4A¹⁰¹³/+. A minimum of 34 germaria from at least 17 females were scored for each genotype. (J) The p-values shown correspond to the comparison of the number of spectrosome-containing cells in Pdcd4¹/Pdcd4¹; bam^Δ86/+ compared to the control genotypes bam^Δ86/+ and Pdcd4¹/Pdcd4¹. A minimum of 37 germaria from at least 18 females were scored for each genotype.