Figure 6
Loss-of-function Pdcd4 mutations result in an increase in the number of pre-cystoblasts. (A-C) Immunofluorescence images showing germaria from 5-day-old females of the indicated genotypes. Immunostaining with pMad (red channel) was used as a marker for GSCs. Anti-Hts (green in A&B) decorated spectrosomes and fusomes and Pdcd4-GFP (the GFP protein trap Pdcd4G93, green channel in C) reported the expression of Pdcd4. Arrowheads indicate examples of high nuclear pMad expression. (D-E) Immunofluorescence images showing single projections of confocal Z-stacks of germaria from 5-day-old females of the indicated genotypes. Immunostaining for bam-GFP (green channel) was used as a marker for CBs, and immunostaining with anti-Hts (red channel) decorated spectrosomes and fusomes. Asterisks indicate the locations of apical tips of the germaria. Scale bars are 10 μm. (F) A schematic depicting the relationship between spectrosome/fusome morphology, pMad and Bam expression, and cell identity within the germarium. Pre-cystoblasts are defined as cells containing spherical spectrosomes that do not express pMad or Bam.