In ovo serial skeletal muscle diffusion tractography of the developing chick embryo using DTI: feasibility and correlation with histology

Table 1 Skeletal muscle FA, length of tracked fibers, length of hind limb and FiberArea% values for chick embryonic hind limb at each day of incubation

Incubation Day(X)	FA ± s.d.	Fiber_length± s.d.(mm).	FiberArea% ± s.d.	Limb_length± s.d.(mm).
Day 5	0.119 ± 0.009	‡	0.465 ± 0.032	1.700±0.274
Day 6	0.138 ± 0.018	‡	0.516 ± 0.029	2.800±0.274
Day 7	0.159 ± 0.024	‡	0.561 ± 0.008	4.200±0.274
Day 8	0.228 ± 0.039	6.542 ± 0.425	0.605 ± 0.015	5.900±0.224
Day 9	0.275 ± 0.026	6.708 ± 0.429	0.631 ± 0.015	6.900±0.224
Day 10	0.284 ± 0.073	8.258 ± 0.829	0.667 ± 0.016	9.000±0.354
Day 11	0.338 ± 0.019	9.654 ± 0.425	0.698 ± 0.013	10.900±0.418
Day 12	0.366 ± 0.019	10.502 ± 0.585	0.718 ± 0.008	12.100±0.224
Day 13	0.431 ± 0.024	11.236 ± 0.308	0.729 ± 0.014	14.200±0.570
Day 14	0.474 ± 0.025	12.110 ± 0.611	0.757 ± 0.006	16.800±0.274
Day 15	0.501 ± 0.044	14.266 ± 0.381	0.776 ± 0.012	18.200±0.447
Day 16	0.511 ± 0.037	15.094 ± 0.769	0.814 ± 0.013	21.100±0.418
Day 17	0.523 ± 0.047	16.576 ± 1.078	0.844 ± 0.021	26.800±0.570
Day 18	0.573 ± 0.088	21.828 ± 1.454	0.867 ± 0.013	30.900±0.418
Model Diagnostics
n	70	55	70	70
R²	0.929	0.908	0.962	0.951
F value	885.825	520.369	1696.561	1313.533
P value	<0.001	<0.001	<0.001	<0.001
Regression Equation	Y₁ = −0.070 + 0.037 X	Y₂ = −5.331 + 1.339 X	Y₃ = 0.357 + 0.029 X	Y₄= −10.999+ 2.084 X

Model diagnostics show linear regression parameters for incubation day (X) dependence of skeletal muscle FA (Y1), length of tracked fibers(Y2), FiberArea% (Y3) and length of hind limb(Y4) values. ‡: No fibers tracked in DTI from day 5 to 7.

ISSN: 1471-213X