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Figure 8 | BMC Developmental Biology

Figure 8

From: In ovo serial skeletal muscle diffusion tractography of the developing chick embryo using DTI: feasibility and correlation with histology

Figure 8

Illustration of the FiberArea% algorithm. (A) Chick embryonic hind limb skeletal muscle micrograph (400×) at incubation day 12. (B) The corresponding micrograph, converted to grayscale. (C) The magnified region of interest (red rectangle) in (B). Example pixel values are: green rectangle, 204, corresponding to background/mesenchymal tissue; red rectangle, 41, corresponding to nucleus; and blue rectangle, 118, corresponding to a myofiber. (D) Pixels belonging to the skeletal muscle fibers only (pixels with values between 90 and 200); all the other pixels value is set to white (value 255).

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